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For calls you place yourself over the calling API, and for web sessions. Campaigns don’t take them.On a web session, the instructions and any variables ride in the token the browser gets. Don’t put secrets there.
Every call an assistant makes runs on the same prompt, the instructions you gave it when you created it. Sometimes one call needs a line the prompt doesn’t have. Per-call instructions are that line: text you send with the call request, appended to the prompt for that call only.

Sending one

A complete call. The variables say who the farmer is. The highlighted line says what is different about today:
A late follow-up, dialed by hand
additionalInstructions takes up to 2,000 characters and lands right after the prompt. The only thing after it is the caller record, when you send variables. Nothing else about the assistant changes, and the text is not returned on any read. The call request reference has the rest of the body.

What to put in it

Steering, not facts. The prompt says who the assistant is and how it works. The instruction says what is different about this one call:
Facts about the caller, like the order number or the crop, belong in variables.

Keep it to a few lines

Say the one thing that is different and stop. A long addition starts competing with the prompt it was meant to nudge.